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Table 2 Candidate reference genes and primer sequences used for quantitative PCR analysis

From: Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions

Gene name

Source gene a

Gene description

Primer sequence b

Amplicon length (bp)

poly

SGN-U347154

hexameric polyubiquitin

5' CGCTGACTACAATATCCAAAAGGA 3'

67

   

5' CTGCATTCCACCCCTCAGA 3'

 

adh

SGN-U350348

alcohol dehydrogenase class III

5' CCTCAAGCCGGCGAAGT 3'

55

   

5' CTGTATGGCAGAGGGCAGTGT 3'

 

actin

SGN-U353034

actin 7

5' AATTGTCCGTGACATCAAGGAA 3'

82

   

5' TGAGCTGCTCTTGGCTGTTTC 3'

 

gapdh

SGN-U347734

glyceraldehyde-3-phosphate dehydrogenase

5' TTGAAGGGCGGTGCAAA 3'

59

   

5' AACATGGGTGCATCCTTGCT 3'

 

rpl7

SGN-U351477

60S ribosomal protein L7

5' CATTCGAGGTATCAATGCTATGCA 3'

66

   

5' TGTCTCAGGCGCAGAAGCT 3'

 

ccs

SGN-U350284

caffeine synthase 1

5' CAATGCCCGGCTCTTTCTAC 3'

68

   

5' GTAACAAGAGTGTAAAAAATGCATGGA 3'

 

cys

SGN-U352616

cysteine proteinase

5' GCGATCGCTACCGTCCAA 3'

63

   

5' CTTTTTCTCTCCAGTCAATGGAGTT 3'

 

14-3-3

SGN-U356404

14-3-3 protein

5' TGTGCTCTTTAGCTTCCAAACG 3'

75

   

5' CTTCACGAGACATATTGTCTTACTCAAA 3'

 
  1. a Unigene accession number according to the SOL Genomics Network [65]
  2. b Forward (upper line) and reverse (lower line) primer sequences
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BMC Molecular Biology

ISSN: 1471-2199

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