Table 2 Details of TaqMan® real-time PCR assays
From: Quantitative TaqMan® real-time PCR assays for gene expression normalisation in feline tissues
Gene | Oligo | Sequence | Amplicon size (bp) | Genomic position (exon) | Genomic (bp) | Detection gDNA | Pseudo-gene |
|---|---|---|---|---|---|---|---|
ABL | Forward | TGTGGCGAGTGGTGATAATACAC | 83 | 2 | ~12,000 7 | No | No |
Probe | CAGCATCACTAAAGGTGAAAAGCTACGAGTCCTT 2 | 2/3 | |||||
Reverse | TCCACTCACCATTCTGGTTGTAA | 3 | |||||
ACTB | Forward | CAACCGTGAGAAGATGACTCAGA | 127 | 3/4 | 410 8 | (Yes) 11 | Yes |
Probe | TCTCTGTACGCTTCTGGCCGCACC 3 | 4 | |||||
Reverse | CCCAGAGTCCATGACAATACCA | 4 | |||||
B2M | Forward | CGCGTTTTGTGGTCTTGGT | 84 | 1 | 3,270 7 | No | No |
Probe | CGGACTGCTCTATCTGTCCCACCTGGA 2 | 1 | |||||
Reverse | AAACCTGAACCTTTGGAGAATGC | 1/2 | |||||
GAPDH | Forward | GCCATCAATGACCCCTTCAT | 82 | 1 | NA | (Yes) 11,12 | Yes |
Probe | CTCAACTACATGGTCTACATGTTCCAGTATGATTCCA 4 | 1/2 | |||||
Reverse | GCCGTGGAATTTGCCGT | 2 | |||||
GUSB | Forward | CTACATCGATGACATCACCATCAG | 80 | 4 | 532 9 | Yes | No |
Probe | ACCAGCGTGAACCAAGACACTGGGC 3 | 4/5 | |||||
Reverse | CGCCTTCAACAAAAATCTGGTAA | 5 | |||||
HMBS | Forward | TGGCAGTGCTGAAAGCCTTA | 94 | 3 | 554 7 | No | No |
Probe | TTGAAATCGTTGCTATGTCCACCACAGG 2 | 3/4 | |||||
Reverse | TTAGAGAGCGCAGTATCAAGAATCTT | 4 | |||||
HPRT | Forward | AACTGGAAAGAATGTCTTGATTGTTG | 100 | 4/5 | >90,000 7 | (Yes) 11 | Yes |
Probe | CACTGGCAAAACAATGCAAACCTTGCTTT 3 | 6 | |||||
Reverse | GACCATCTTTGGATTATACTGCTTGA | 6 | |||||
RPS7 | Forward | GTCCCAGAAGCCGCACTTT | 74 | 4/5 | ~2,200 10 | (Yes) 11 | Yes |
Probe | CGCCGTGCACGACGCGA 5 | 5 | |||||
Reverse | CACAATCTCGCTCGGGAAAA | 5 | |||||
YWHAZ | Forward | ACAAAGACAGCACGCTAATAATGC | 84 | 4 | 2,878 7 | (Yes) 11 | Yes |
Probe | CGATGTCCACAATGTCAAGTTGTCTCTCAGTAAT 3 | 4/5 | |||||
Reverse | CTTCAGCTTCATCTCCTTGGGTAT | 5 | |||||
18S rRNA 1 | Forward | CGGCTACCACATCCAAGGAA | NA | NA | NA | NT | NT |
Probe | TGCTGGCACCAGACTTGCCCTC 6 | NA | |||||
Reverse | GCTGGAATTACCGCGGCT | NA |