Table 3 Primers used for production of standard templates
From: Quantitative TaqMan® real-time PCR assays for gene expression normalisation in feline tissues
Gene | Forward Primer Reverse Primer | Sequences | Annealing Temperature (°C) | Amplification product (bp) |
|---|---|---|---|---|
ABL | fABL_StdF | GGCTTTGAGGGAGACAAGAC | 62 | 402 |
fABL_StdR | GAAGCTGCCATTGATCAGAC | |||
ACTB | fACTB_StdF | CCATCGAACACGGCATTGTCAC | 58 | 431 |
fACTB_StdR | CTTGATGTCACGCACAATTTCCCG | |||
B2M | fB2M-F | GGCGCGTTTTGTGGTCTTGGTC | 63 | 339 |
fB2M-R | CACTTAACGACCTTGGGCTC | |||
GUSB | fGUSB_StdF | GCCGCATTACCATTGCCATCAAC | 64 | 384 |
fGUSB_StdR | GCATCAGGTATGGCCACCAGAG | |||
HMBS | fHMBS_StdF | CAGCCCAAAGATGAGAGTGATTCG | 64 | 339 |
fHMBS_StdR | GGGTGAAAGACAACGGCATCATAG | |||
RPS7 | fRPS7-F | AGCTGAGGGAGCTGAACATC | 65 | 432 |
fRPS7-R | TGCCCGTGAGCTTCTTATAG | |||
YWHAZ | fYWHAZ_StdF | GAGGTTGCTGCTGGTGATGAC | 64 | 329 |
fYWHAZ_StdR | CCTGCTTCAGCTTCATCTCCTTGG |