Table 4 Optimal final concentration of primers and probe for the newly designed real-time PCR assays
From: Quantitative TaqMan® real-time PCR assays for gene expression normalisation in feline tissues
Real-time PCR assay | Forward Primer (nM) | Reverse Primer (nM) | Probe (nM) |
|---|---|---|---|
ABL | 300 | 900 | 50 |
ACTB | 900 | 900 | 50 |
B2M | 300 | 900 | 100 |
GUSB | 900 | 300 | 100 |
HMBS | 900 | 900 | 250 |
HPRT | 900 | 900 | 50 |
RPS7 | 50 | 900 | 200 |
YWHAZ | 900 | 900 | 150 |