Table 5 Ranking of potential reference genes according to the expression stability
From: Quantitative TaqMan® real-time PCR assays for gene expression normalisation in feline tissues
Tissues | Analsysis | Ranking |
|---|---|---|
All endocrine tissues tested | NF | YWHAZ > GUSB > B2M > RPS7> HMBS > ABL > 18S > GAPDH > ACTB > HPRT |
gN(M) | B2M > GUSB > YWHAZ > HMBS > RPS7 > ABL > 18S > GAPDH > ACTB > HPRT | |
gN | B2M = HMBS > GUSB > YWHAZ > RPS7 > ABL > 18S > GAPDH > ACTB > HPRT | |
Adrenal gland | NF | HPRT > RPS7 > ABL > B2M > ACTB > GAPDH > 18S > HMBS > YWHAZ > GUSB |
gN(M) | HPRT > RPS7 > ABL > ACTB > B2M > 18S = GAPDH > HMBS > YWHAZ > GUSB | |
gN | GAPDH = 18S > RPS7 > HPRT > YWHAZ > ABL > B2M > ACTB > HMBS > GUSB | |
Pancreas | NF | RPS7 > GUSB > YWHAZ > HMBS > B2M > GAPDH > ABL > 18S > HPRT > ACTB |
gN(M) | RPS7 > GUSB > YWHAZ > B2M > HMBS > ABL > GAPDH > 18S > HPRT > ACTB | |
gN | GUSB = RPS7 > YWHAZ > HMBS > B2M > ABL > 18S > GAPDH > HPRT > ACTB | |
Parathyroid | NF | ACTB > RPS7 > GAPDH > HMBS > YWHAZ > ABL > B2M > GUSB > 18S > HPRT |
gN(M) | ACTB > RPS7 > GAPDH > ABL > HMBS > YWHAZ > B2M > GUSB > 18S > HPRT | |
gN | GAPDH = RPS7 > ACTB > HMBS > ABL > YWHAZ > B2M > GUSB > 18S > HPRT | |
Thyroid | NF | GUSB > ACTB > YWHAZ > HMBS > B2M > RPS7 > ABL > 18S > GAPDH > HPRT |
gN(M) | ACTB > GUSB > YWHAZ > B2M > HMBS > RPS7 > ABL > 18S > GAPDH > HPRT | |
gN | ACTB = HMBS > GUSB > B2M > YWHAZ > RPS7 > ABL > 18S > GAPDH > HPRT | |
All lymphoid tissues tested | NF | RPS7 > GUSB > ACTB > YWHAZ > ABL > GAPDH > B2M > HPRT > 18S > HMBS |
gN(M) | GUSB > RPS7 > ACTB > YWHAZ > ABL > B2M > GAPDH > HPRT > 18S > HMBS | |
gN | GUSB = RPS7 > ACTB > YWHAZ > ABL > B2M > GAPDH > HPRT > 18S > HMBS | |
Bone marrow | NF | RPS7 > HMBS > ABL > GUSB > YWHAZ > ACTB > B2M > 18S > HPRT > GAPDH |
gN(M) | RPS7 > HMBS > ABL > GUSB > YWHAZ > ACTB > B2M > 18S > HPRT > GAPDH | |
gN | ACTB = YWHAZ > HMBS > GUSB > RPS7 > ABL > B2M > 18S > HPRT > GAPDH | |
Lymph node | NF | GUSB > ACTB > B2M > HMBS > YWHAZ > ABL > RPS7 > GAPDH > 18S > HPRT |
gN(M) | GUSB > B2M > ACTB > YWHAZ > ABL > HMBS > RPS7 > GAPDH > 18S > HPRT | |
gN | ABL = B2M > YWHAZ > ACTB > GUSB > HMBS > GAPDH > RPS7 > 18S > HPRT | |
Spleen | NF | GUSB > RPS7 > ACTB > ABL > B2M > HMBS > YWHAZ > HPRT > GAPDH > 18S |
gN(M) | GUSB > RPS7 > ACTB > B2M > ABL > HMBS > YWHAZ > HPRT > GAPDH > 18S | |
gN | ACTB = B2M > GUSB > RPS7 > ABL > HMBS > YWHAZ > HPRT > GAPDH > 18S | |
All gastrointestinal tissues tested | NF | ACTB > HMBS > YWHAZ > RPS7 > ABL > GAPDH > GUSB > B2M > 18S > HPRT |
gN(M) | ACTB > HMBS > YWHAZ > RPS7 > ABL > GAPDH > GUSB > B2M > 18S > HPRT | |
gN | ABL = HMBS > RPS7 > ACTB > YWHAZ > GAPDH > B2M > GUSB > 18S > HPRT | |
Parotid gland | NF | ABL > ACTB > RPS7 > YWHAZ > HMBS > B2M > HPRT > GAPDH > GUSB > 18S |
gN(M) | ABL > RPS7 > ACTB > YWHAZ > HMBS > B2M > HPRT > GAPDH > GUSB > 18S | |
gN | ABL = ACTB > HMBS > RPS7 > YWHAZ > B2M > HPRT > GAPDH > GUSB > 18S | |
Duodenum | NF | RPS7 > GAPDH > ACTB > HMBS > YWHAZ > ABL > 18S > GUSB > B2M > HPRT |
gN(M) | RPS7 > GAPDH > ACTB > HMBS > YWHAZ > ABL > 18S > GUSB > B2M > HPRT | |
gN | ACTB = HMBS > RPS7 > GAPDH > YWHAZ > ABL > 18S > GUSB > B2M > HPRT | |
Ileum | NF | HMBS > ABL > YWHAZ > ACTB > GAPDH > GUSB > RPS7 > HPRT > B2M > 18S |
gN(M) | HMBS > ABL > ACTB > YWHAZ > GAPDH > GUSB > RPS7 > HPRT > B2M > 18S | |
gN | YWHAZ = ACTB > HMBS > ABL > GUSB > GAPDH > RPS7 > HPRT > B2M > 18S | |
Liver | NF | GUSB > GAPDH > RPS7 > HPRT > HMBS > YWHAZ > ABL > ACTB > B2M > 18S |
gN(M) | GAPDH > HMBS > HPRT > GUSB > RPS7 > YWHAZ > ABL > ACTB > B2M > 18S | |
gN | ACTB = HMBS > ABL > GAPDH > HPRT > GUSB > YWHAZ > RPS7 > B2M > 18S | |
Kidney | NF | YWHAZ > RPS7 > ABL > ACTB > HMBS > GUSB > GAPDH > B2M > HPRT > 18S |
gN(M) | YWHAZ > RPS7 > ABL > ACTB > HMBS > GUSB > GAPDH > B2M > HPRT > 18S | |
gN | RPS7 = YWHAZ > ABL > ACTB > HMBS > GAPDH > GUSB > B2M > HPRT > 18S | |
Myocardium | NF | ACTB > RPS7 > GAPDH > HMBS > YWHAZ > ABL > GUSB > 18S > HPRT > B2M |
gN(M) | RPS7 > ACTB > GAPDH > YWHAZ > HMBS > ABL > GUSB > 18S > HPRT > B2M | |
gN | GAPDH = YWHAZ > RPS7 > ACTB > HMBS > ABL > GUSB > 18S > HPRT > B2M | |
Brain | NF | ACTB > ABL > RPS7 = YWHAZ > HMBS > GAPDH > HPRT > GUSB > B2M > 18S |
gN(M) | ACTB > ABL > RPS7 > YWHAZ > HPRT > GAPDH > HMBS > GUSB > B2M > 18S | |
gN | ABL = RPS7 > ACTB > GAPDH > HPRT > YWHAZ > HMBS > GUSB > B2M > 18S | |
All healthy tissues combined | NF | RPS7 > GUSB > YWHAZ > ABL > ACTB > B2M > HMBS > 18S > GAPDH > HPRT |
gN(M) | RPS7 > GUSB > YWHAZ > ABL > ACTB > B2M > HMBS > 18S > GAPDH > HPRT | |
gN | GUSB = RPS7 > ABL > YWHAZ > ACTB > B2M > HMBS > 18S > GAPDH > HPRT | |
Blood | NF | YWHAZ > ABL > GAPDH > B2M > ACTB > GUSB > 18S > HPRT > RPS7 > HMBS |
gN(M) | YWHAZ > B2M > GAPDH > ABL > ACTB > 18S > GUSB > HPRT > RPS7 > HMBS | |
gN | YWHAZ = B2M > ACTB > 18S > GAPDH > ABL > GUSB > HPRT > RPS7 > HMBS | |
Neoplastic tissues | NF | RPS7 > ACTB > HMBS > GAPDH > B2M > ABL > 18S > GUSB > YWHAZ > HPRT |
gN(M) | ACTB > RPS7 > HMBS > GAPDH > B2M > 18S > ABL > GUSB > YWHAZ > HPRT | |
gN | GAPDH = HMBS > 18S > RPS7 > ACTB > B2M > ABL > GUSB > YWHAZ > HPRT |