Figure 3
Map of episomal Hermes integration sites in the target plasmid pGDV1. The x axis refers to the linear form of the target plasmid and the position of the chloramphenicol resistance gene (CamR) is shown. The numbers on the x-axis refer to the length of the plasmid in basepairs. The y-axis is the number of integrations. Positive and negative values refer to elements that integrated into the target in opposite orientations. Five different classes of Hermes episomes were tested for their ability to integrate with each differing only in the amount of intercalary DNA (n) located between the inverted terminal repeats. The unweighted consensus sequence of the Hermes target site is graphically represented with a sequence logo [58] constructed with WebLogo [59].