Table 5 Antibodies, sources, and working concentrations used in immunohistochemistry.
Antibodies | Catalog and Source | Species | Working Concentration |
|---|---|---|---|
CPEB3, polyclonal | Ab10883 Abcam, Cambridge, MA | Rabbit | 1:50 |
Map1a, monoclonal | M4278 Sigma,, St. Louis, MO | Mouse | 1:200 |
ChAT, polyclonal | AP144-p Millipore, Bedford, MA | Goat | 1:200 |
Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) | A11034 Invitrogen, Carlsbad, CA | Donkey | 1:200 |
Alexa Fluor® 594 donkey anti-mouse IgG | A21203 Invitrogen, Carlsbad, CA | Donkey | 1:200 |
Alexa Fluor® 594 donkey anti-rabbit IgG (H+L) | A21207 Invitrogen, Carlsbad, CA | Donkey | 1:200 |
Alexa Fluor® 488 donkey anti-goat IgG | A11055 Invitrogen, Carlsbad, CA | Donkey | 1:200 |