Figure 4From: Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86Alignment of the amino acid sequences of the Bm86 homologues from ticks for which Bm86 vaccine efficiency has been documented: Bm86 AUS (Australian strain) (AAA30098), Bm86 MOZ (Mozambique strain) (FJ809946), Ba86 (ABY58969), Bd86 (ABG21131), Haa86 (AAL36024), Ra86-1 (FJ809944) and Ra86-2 (FJ809945). Cross reactive linear B-cell epitopes mapped using pin-coupled peptides by Odongo et al. [5] are boxed, the regions identified using biotin-coupled peptides by the same authors are underlined. Three synthetic peptides used by Patarroyo et al. [33] which induced an immune response against R. microplus are double underlined and EGF-like domains fitting the pattern Cys-Xaa48-Cys-Xaa3-6-Cys-Xaa8-11-Cys-Xaa0-1-Cys-Xaa5-15-Cys (where Xaa is any amino acid except for cysteine) with 5 or 6 cysteine residues are shaded grey. The phenylalanine at position 507 of Bm86 AUS was predicted to be a cysteine when the sequence of a second cDNA clone from a separate library was determined by Rand et al. [12].Back to article page