Figure 5

Genetic characterization and expression studies of Rv1417 and Rv2617c. A. Distribution of Rv1417 and Rv2617c in species of the MTC. PCR amplifications of Rv1417 (lanes 1–18) and Rv2617c (lanes 19–36) were performed with pairs of primers 1417pktup/1417pktlow and 2617pktup/2617pktlow (table 1), respectively, and using the following genomic DNA as template: lanes 2 and 20, M. tuberculosis H37Rv; lanes 3–7 and 21–25, M. microti isolates; lanes 8–12 and 26–30, M. pinnipedii isolates; lanes 13–17 and 31–35, M. bovis isolates; lanes 18 and 36, M. caprae isolate. PCR negative controls were included (lanes 1 and 19). Arrows indicate the size of the bands. B. Transcription of Rv1417 and Rv2617c in M. tuberculosis. The transcription of Rv1417 (lanes 1–4) and Rv2617c (lanes 5–8) was studied by RT-PCR assays using the pairs of primers 1417pktup/1417pktlow and 2617pktup/2617pktlow (table 1), respectively. Lanes 1 and 5, PCR negative controls; lanes 2 and 6, M. tuberculosis DNA (positive control); lanes 3 and 7, RT-PCR amplifications without RT; lanes 4 and 8, RT-PCR amplifications with RT. Arrow indicates the size of the bands. C. Genomic organization of Rv1417 homologous loci in Actinomycetales. Schematic representation of genes encoding conserved proteins in the neighbourhood of Rv1417 in Actinomycetales genomes. Genes encoding homologous proteins are depicted in colours or patterns. Comparative genomic analysis was carried out with the STRING software and BLASTP analysis of the protein sequences deduced from genomic data bases.