Figure 3From: Spinocerebellar ataxia type 8 larger triplet expansion alters histone modification and induces RNA fociATXN8OS CR lines ChIP-PCR assay using anti-dimethyl H3 (K9) and anti-acetyl-histone H3 (K14) antibodies. ATXN8OS CR cells were sonicated to shear DNA. DNA was then isolated, precipitated with anti-dimethyl H3 (K9) (A) or anti-acetyl-histone H3 (K14) (B) antibody, and used as template for PCR amplification of 213-bp exon B to A-containing fragment. Lanes P represents positive control.Back to article page