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Figure 3 | BMC Molecular Biology

Figure 3

From: A single hydrophobic cleft in the Escherichia coli processivity clamp is sufficient to support cell viability and DNA damage-induced mutagenesis in vivo

Figure 3

Steady-state levels of mutant β clamp proteins. Steady-state levels of the different clamp proteins were examined as described in 'METHODS.' As noted in 'METHODS,' we previously determined the number of clamp proteins expressed in E. coli MS101 bearing either pWSK29, or pWSK29-derived plasmids expressing different clamp proteins [27]. Note that both β159 and βC-G66E possess altered mobility in SDS-PAGE relative to the wild-type clamp, or the other mutants, due to the G66E substitution [24].

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