Figure 4
Ability of βC to complement the temperature-sensitive growth phenotype of the dnaN159 strain. Serial dilutions of strains MS101 (dnaN159), MS125 [dnaN159 Δ(dinB-yafN)::kan], MS146 [dnaN159 Δ(araD-polB):: Ω], and MS147 (dnaN159 ΔumuDC595::cat) bearing plasmids directing physiological levels of expression of the indicated clamp proteins were spotted onto Luria-Bertani agar plates supplemented with Amp and IPTG. Plates were photographed following overnight incubation at 30° or 42°C, as indicated. At least two independent transformants for each strain were examined. Representative results are shown. 'Control' refers to the strain bearing the empty pWSK29 plasmid. See Table 1 for details concerning the E. coli strains and plasmids used.