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Table 3 Comparison of the ranking of B2M and ActB, the selected chromosomal reference genes, and Rluc, present on a transiently co-transfected plasmid, based on geNorm, Normfinder and BestKeeper analyses

From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells

 

geNorm

Normfinder

BestKeeper

 

M value

Ranking

Stability value

Standard error

Ranking

[r]

p-value

Ranking

ActB

0.44

1/2

1.342

0.515

3

0.931

0.001

2

B2M

0.44

1/2

0.432

0.955

1

0.985

0.007

1

Rluc

0.63

3

1.236

0.507

2

0.694

0.001

3

  1. Highlighted regions represent the best ranked gene in the analysis. Abbreviations: nd- not determined; M value- geNorm measure of expression stability, [r]- BestKeeper coefficient of correlation, p-value- BestKeeper probability value
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BMC Molecular Biology

ISSN: 1471-2199

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