Table 8 Nucleotide sequences of the primers used in the qPCR reactions and the length of the amplicons generated
Transcript (amplicon length) | Primer | Sequence (5' to 3') |
|---|---|---|
Fluc (137 nts) | prMJ296 | AGGTGGCTCCCGCTGAAT |
prMJ297 | FAM-CGGGAAGACCTGCGACACCTGCGT-BHQ1 | |
prMJ298 | CATCGTCTTTCCGTGCTCCA | |
Rluc (140 nts) | prMJ274 | GCAGAAGTTGGTCGTGAGG |
prMJ272 | HEX-CTCACTATAGGCTAGCCACCATGACTTCGAAAG-BHQ1 | |
prMJ276 | TCATCCGTTTCCTTTGTTCTG | |
B2M (125 nts) | prMJ348 | TCTCTGCTCCCCACCTCTAA |
prMJ349 | FAM-CCAGCCCTCCTAGAGCTACC-BHQ1 | |
prMJ350 | ATCTGAGCAGGTTGCTCCAC | |
ActB (142 nts) | prMJ351 | CTCGGCCACATTGTGAACTT |
prMJ352 | FAM-ATGCTCGCTCCAACCGAC-BHQ1 | |
prMJ353 | AACGGTGAAGGTGACAGCA |