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Table 9 The efficiencies and errors of the qPCR standard curves as calculated by the Lightcycler 480 software (Roche Diagnostics Ltd, Burgess Hill, UK)

From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells

Standard curve

Efficiency

Error

Fluc

1.878

0.001

Rluc

1.878

0.001

B2M

2.027

0.053

ActB

1.994

0.002

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BMC Molecular Biology

ISSN: 1471-2199

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