Figure 4
From: Inflammation in the avian spleen: timing is everything
Quantitative RTPCR analysis of cytokine gene expression in the spleen. Plotted dark circles represent the mean ± SEM in each experimental group. The dashed line represents the fitted plot of cosinor analysis utilizing linear harmonic regression. Values are represented as the number of transcript copies/1000 GAPDH transcripts. Abscissa labels indicate ZT time values every 3 hrs under DD conditions; crosshatched indicates darkness. TNFά became statistically arrhythmic at a >2-fold change while IL-1β (pANOVA = .004; pcosinor = .003) and IL-6 (pANOVA < .001; pcosinor = .003) mRNAs maintained robust circadian oscillations with maximal levels occurring at subjective midnight and subjective dawn, respectively. IL-2 (pANOVA < .001; pcosinor = .003), IL-l2β (pANOVA < .001; pcosinor = .002) and IL-18 (pANOVA < .003; pcosinor = .006) revealed that the mRNAs for each reached maximal values at early subjective day.