Figure 3
From: Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
![Figure 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2199-11-14/MediaObjects/12867_2009_Article_495_Fig3_HTML.jpg)
SRF and Elk-1 bind to the ZC3H12A promoter. A, Gel retardation assay with a fragment of ZC3H12A promoter (-95 - +67) containing the WT sequence or a mutated CArG box (ZC3H12A-mut) or a fragment of c-Fos promoter. The DNA was incubated with coreSRF. B and C, Chromatin immunoprecipitation of the transcription factors Elk-1 (B) and SRF (C) bound to the ZC3H12A promoter. HepG2 cells were incubated in 0.5% FCS medium for 12 hrs and then stimulated for 30 min with IL-1β. The antibodies anti-Elk-1 (B) or anti-SRF (C) were used to immunoprecipitate sonicated chromatin. As a negative control non-specific IgG were employed. Eluted DNA served as a template in PCR analysis with oligonucleotides specific for ZC3H12A promoter (top panel B and C) or EGR promoter (bottom panel B and C). 1% of input DNA is shown in lines 5 and 6. The panels show the inverted images of ethidium bromide-stained gels.