Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

Table 1 Selected candidate reference genes, primers and different parameters derived from qRT-PCR analysis

Gene name	GenBank accession number	Primer sequences (forward/reverse)	Tm (°C)	Amplicon length (bp)	Amplification efficiency (%)	*S.D of efficiency	Average Cp of cDNA	**R²
ACT	EF528575	CCAAATCCAGCTCATCAGTCG TCTTTCGGCTCCGATGGTGAT	80.26	74	94.31	0.123	24.29	0.9992
TUB	AF101419	GCACGGCATTGATGTGACC GAACAAACCTCCCGCCACT	82.56	101	95.77	0.0094	20.65	0.9995
NADHD	L39390	TGCAGCAAAGGCTTGTCAAA TCGAAACTTCCCGTTATCCAA	75.84	102	84.89	0.0104	27.12	0.9989
EF	AY378166	CATGCGTCAGACGGTTGCTGT CTTCACTCCCTTCTTGGCTGC	82.17	100	98.17	0.0055	19.11	0.9999
H3	AY378165	ACAGCTCGCAAATCAACCG GCGGCTTCTTCACTCCACC	83.79	100	94.49	0.0059	26.46	0.9998
rRNA	U42501	GGCGACGCATCATTCAAAT TCCGGAATCGAACCCTAAT	80.61	102	91.27	0.0107	7.11	0.9992
GAPDH		AGGGCGGTGCTAAGAAAGTCA TCTGGCTTGTATTCCTTCTCATT	81.55	91	91.04	0.0037	29.58	0.9999
FEHIIa/FEHIIb	AY323935/AJ295034.1	TAAAGACTTGAAAGAACAAAGTG CGCACCATAACTTGTCGTGTCG	78.98	135	82.24	0.0116	31.53	0.9981

*S.D, standard deviation; **R², correlation coefficient of the slope of the standard curve

ISSN: 1471-2199