Figure 3
From: Non-consensus GLI binding sites in Hedgehog target gene regulation
Validation of the transcriptional activation obtained with the PTCH1_VAR construct containing a selection of different binding site variants with a modified PTCH1_WT construct in a luciferase assay. GLI binding site variations (3T, 8A, 6G7G) were introduced into the PTCH_WT construct by site directed mutagenesis. HaCaT cells were co-transfected with the indicated reporter constructs and a GLI2act expression construct together with LacZ for normalization. No significant differences were observed between the mutated PTCH_WT construct and the PTCH_VAR construct demonstrating the relevance of the PTCH_VAR test system. 6G7G was used as inactive negative control.