Skip to main content
Figure 5 | BMC Molecular Biology

Figure 5

From: Non-consensus GLI binding sites in Hedgehog target gene regulation

Figure 5

Changing functional non-consensus sites to consensus sites in selected GLI target gene promoters does not enhance transcription. Wild type or mutated luciferase reporter constructs were co-transfected into HaCaT cells with GLI2act expression construct or empty expression vector (pcDNA4/to) as control. (A) The human wild type JUN promoter (JUNprom_WT, upper panel) contains a functional binding site with two substitutions compared to the GLI consensus sequence (2G5C) (upper panel). The GLI consensus sequence, when introduced into this site (JUNprom_CONS), showed comparable reporter gene activation to the variant 2G5C. (B) The human GLI1 promoter contains a functional binding site with the variant nucleotide G at position 9 (GLIprom_WT, upper panel). Introduction of the consensus sequence at this position led to slightly higher activation in response to GLI2act than the wild type sequence.

Back to article page