BMC Molecular Biology

Table 1 Random mutagenesis of residues between 493-512 in the mCry2-T4 construct and relative luciferase results.

From: Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

																					Luciferase Assay
mCry2-T4	E	T	S	R	L	N	I	E	R	M	K	Q	I	Y	Q	Q	L	S	R	Y	+++++
M1					P		T		Q				T		X		P				-
M2	G				P				Q		R		T				P		R		-
M3					P		T						T		X		P				++
M4			G		P		T						T						R		+++
M5			G		P		T		Q		R		T				P		R		-
M6	G				P		T		Q				T				P				-
M7							T		Q												-
M8							T				R		T				P				-
M9	G		G		P		T						T						R		++
M10					P		T						T				P				+++
M11	G				P						R		T				P				-
M12					P		T						T						R		++
M13			G		P		T		Q		R		T				P		R		-
M14			G		P		T				R		T				P				-
M15							T		Q				T				P		R		-
M16					P		T		Q				T				P				-
M17										T											++++
M18				Q		D				T								P			++++
M19								G		T								P			++++
M20				Q										H						H	++++
M21		I		Q		D				T				H		X		P		H	+++
M22				Q		D				T				H				P			++++
M23				Q								X		H				P		H	++
M24		I								T				H		X					+
M25								G						H						H	+
M26				Q		D				T				H		X				H	++

Only mutated positions are listed. × indicates the stop codon.

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ISSN: 1471-2199

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