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Figure 2 | BMC Molecular Biology

Figure 2

From: Effects on mitochondrial transcription of manipulating mTERF protein levels in cultured human HEK293 cells

Figure 2

Manipulation of mTERF expression has minimal effects on levels of mature mitochondrial RNAs during recovery from EtBr-induced depletion. Relative expression of mitochondrial transcripts in cells overexpressing mTERF, based on phosphorimaging of Northern blots probed successively for mitochondrial tRNAPhe and tRNALeu(UUR). Data (means ± SD) are ratios of tRNAPhe to tRNALeu(UUR) (F/L) normalized to the ratio at the start of the experiment (time-point –2 d). (A) Cells stably transfected with empty-vector (as shown in Additional File 1, Fig. S1B) or mTERF overexpression (OE) construct (clone 3, as shown in Additional File 1, Fig. S1). Overexpressor clones 1 and 2 gave similar results: sample blots shown in Additional File 1, Fig. S2A. (B) Cells treated with mTERF-specific siRNA (or mock-transfected) prior to the addition of EtBr (day –2) and again 2 days after removal of EtBr (day 2). Days 1-5 indicate the period of subsequent recovery. For equivalent data on ratio of tRNAPhe to 5S rRNA from the same experiment see Additional File 1, Fig. S2B, C.

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