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Figure 1 | BMC Molecular Biology

Figure 1

From: Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill

Figure 1

Stages of in vitro adventitious rooting in Eucalyptus globulus. After seeding, seedlings remained 14 weeks in germination medium. After this time, apical microcuttings were obtained and used in adventitious rooting experiments. The microcuttings were kept in induction medium (presence or absence of 10 mg l-1 IBA) for 12, 24, 48 or 96 h, depending on the harvest time. For the harvest points in formation medium, the microcuttings remained 96 h in induction medium and after that were transferred to formation medium (devoid of auxin and with activated charcoal). In this case, the samples were collected after 24 and 48 h. The samples harvested at 12, 24, 48 and 96 h of induction step and at 24 and 48 h of formation step were submitted to RNA extraction and used for the analyses.

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