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Table 2 Primer sequences and amplicon characteristics for each of the 11 reference genes and for the Argonaute 1 gene of interest.

From: Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill

Gene

symbol

Primer sequence (5' → 3')

Forward/Reverse

Amplicon

length (bp)

Annealing

temperatures

(°C)

Amplification

efficiency

18S

TGACGGAGAATTAGGGTTCG/

CCGTGTCAGGATTGGGTAAT

100

60

1.860

ACT2

TCCACCATGTTCCCTGGTAT/

ACCTCCCAATCCAGACACTG

124

60

1.935

EF2

GCGTTCCCTCAGTGTGTCTT/

GGTCATCTGCTCCTTCAAGC

126

60

1.790

EUC12

GCGTGGTTCTTGGATCACTA/

TGGTGACAAAGTCAGGTGCT

114

59

1.807

H2B

GAAGAAGCGGGTGAAGAAGA/

GGCGAGTTTCTCGAAGATGT

145

60

1.972

IDH *

CTGTTGAGTCTGGAAGATGAC/

CATTTAATTCCTCCCCAACAAA

271

60

1.892

UBI

AGAAGGAATCGACCCTCCAC/

CCTTGACGTTGTCAATGGTG

126

60

1.888

SAND

CCATTCAACACTCTCCGACA/

TGTGTGACCCAGCAGAGTAAT

143

59

1.919

TIP41

GAACAAAAGCTCGGGACATC/

CAACCAGCAAGAGCATCAAA

122

60

1.938

TUA

ACCGGTTGATCTCTCAGGTG/

TAAGGGACCAGGTTGGTCTG

103

60

1.845

33380

TCCAGAGTGCAATGCTGAAC/

CCCCTCGTCTGGCATACTTA

134

60

1.935

AGO1

TCTTGGGCTCGTTTCTCAGT

GGAATTCGCCTAGACAGTGC

144

60

1.887

  1. * Primer obtained from Carvalho et al. (2008) [55].