Figure 3

PCR amplification of LYP1 and LYP3 and verification of PCR products by DNA sequencing. A. Single-strand cDNAs obtained from peripheral blood of 11 individuals were amplified with primers L1and L1r which give rise to both LYP1 and LYP3. PCR products were resolved on 1.5% agarose gels and were detected by staining with ethidium bromide. B. and C. LYP1 and LYP3 PCR products shown in panel A above were purified by using 8% polyacrylamide gel and re-amplified with primers L1 and L1r for sequencing analyses with primer L1. Note that underlined sequence of LYP1 in panel B denotes the 84 bp segment missing in LYP3. The sequencing data represent all LYP1 and LYP3 bands shown in Fig. 3A and Fig. 4. D. LYP3 was amplified from the 11 blood cDNA samples shown above in panel A with primers L2 and L1r. PCR products were resolved on 1.5% agarose gels and were detected by staining with ethidium bromide.