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Figure 2 | BMC Molecular Biology

Figure 2

From: Regulation of CEACAM1 transcription in human breast epithelial cells

Figure 2

CEACAM1 promoter regions protected through binding of transcription factors. Top, In vivo footprinting with LM-PCR performed with DNA isolated from DMS-treated MDA-MB-468, MCF10A and MCF7 cells. Top left, footprinting on the coding DNA strand; top right, footprinting on the non-coding DNA strand. G+A, a Maxam-Gilbert sequencing reaction performed with DNA isolated from MDA-MB-468 cells. G, purified genomic DNA from MDA-MB-468 cells modified with DMS in vitro and subjected to LM-PCR. Closed circle, protected bases on the coding strand; open circle, protected bases on the non-coding strand. Bottom, part of the sequence of the proximal CEACAM1 promoter, from -70 bp to -250 bp. Underlined are previously identified binding sites for USF, -143-148, SP1, -153-162, and IRF1, -210-230. Closed and open circles show the protected bases identified by the in vivo footprinting experiment.

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