Figure 5

Chromatin structure at the CEACAM1 promoter in MDA-MB-468, MCF10A and MCF7 cells. A. Chromatin immunoprecipitations of CEACAM1 promoter DNA from MDA-MB-468, MCF10A and MCF7 cells with antibodies detecting acetylated Lys9 and Lys 18 of histone H3 (H3Ac), trimethylated Lys 9 of histone H3 (H3KMe), and trimethylated Lys 27 of histone H3 (H3K27Me). IgG was used as negative control; In: input DNA. B. MCF7 cells were treated with either DMSO or Trichostatin A for 0 h, 6 h, and 24 h, respectively, and total RNA was isolated and subjected to RT-PCR with primers amplifying CEACAM1 (top). GAPDH was used as a loading control. Bottom, CEACAM1 mRNA levels were quantified by real time PCR, using GAPDH for normalization.