Figure 10

H4K16ac is enriched at 3 × UAS -arm-lacZ and UAS -lacZ reporter genes in the presence of Gal4-MOF. Flies carrying the hsp83-Gal4[DB] or hsp83-Gal4-MOF transgenes were crossed to 3 × UAS-arm-lacZ lines or UAS-lacZ. Chromatin from third instar larval offspring was immunoprecipitated with antibody against H4K16ac. The fold enrichment of immunoprecipitated DNA relative to input DNA is shown. Fold enrichment is normalized to the autosomal Gpdh, which is set to 1. A 3-4 fold enrichment is observed for transcribed regions of the control X-linked genes Pgd and arm. Three primer sets were used to amplify different regions within the lacZ gene. A 3 to 7-fold enrichment is observed for the lacZ gene in the lines expressing Gal4-MOF compared to lines expressing Gal4[DB]. A schematic illustration of the 3 × UAS-arm-lacZ and UAS-lacZ reporter genes with relative location of primer pairs used to amplify lacZ sequences is shown below each graph. Arrows indicate the transcription start sites.