Figure 6

DsRed.T4-NLS RNA levels in larvae that express Gal4-MOF fusion proteins. Quantitative real time RT-PCR was used to determine the levels of DsRed RNA in samples from sex-sorted larvae. pka RNA levels were used for normalization. Three biologically independent experiments were performed and each sample was assayed in triplicate. Mean and standard deviation are shown. The DsRed RNA levels in the Gal4-MOF and Gal4-MOF mutant lines are plotted relative to the Gal4[DB] control.