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Figure 2 | BMC Molecular Biology

Figure 2

From: Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals

Figure 2

Human lung TSS I generates a transcript with a 5'-UTR containing an intron of >5 kb. A. A diagram showing the locations of all human primers used in Figure 2 and in following PCR experiments. B. Reverse transcriptase (rt) -PCR analysis of human lung RNA was performed as described in Methods using primers 0 and 14 (shown in A. and in Table 1). C. DNA sequencing was done to demonstrate the position of a 5939 base intron removed from the transcript. Numbering is as in Figure 1. The sequence shown is a portion of the full 80 bp band of 2B.

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BMC Molecular Biology

ISSN: 1471-2199

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