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Figure 6 | BMC Molecular Biology

Figure 6

From: Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals

Figure 6

Transcription from PURA TSS II is decreased when cotransfected with IRF expression plasmids. A. HEK 293T cells were transfected with 0.5 μg of PURA reporter plasmid, and 4 μg of the expression plasmids pEGFP-C1 (hIRF-1, hIRF-3, hIRF-5, hIRF-7, or hIRF-9) or the control plasmid pEGFP-C1. At twenty-four hours post-transfection cells were viewed by fluorescent microscopy for the expression of GFP. At forty-eight hours post-transfection cells were lysed and analyzed for promoter expression as described in Methods. Asterisks indicate a significant difference between transcription from pGL3-hPURAPr and pGL3-hPURAPr Δ((-1514 -1202) in the presence of the overexpressed IRF protein compared to vector. B. The transcription of pGL3-hPURAPr and pGL3-hPURAPr Δ((-1514 -1202) in the presence of the overexpressed IRF proteins is normalized to their transcription in the presence of vector, which is set at 100. Asterisks indicate a significant difference between pGL3-hPURAPr and the deletion construct. All statistical analyses were done with two-way ANOVA using the Bonferroni posttest. ***, P, < 0.001; **, P < 0.01; *, P < 0.05 as conducted using Prism 4.0.

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BMC Molecular Biology

ISSN: 1471-2199

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