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Table 1 Primers used in PCR and RT-PCR

From: Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals

Location

Primer

F/R

species

5'base

3'base

5'- to -3'

TSSI

0

F

Hs

-6131

-6115

CCCGCAGAGCCTAAAGTGAG

TSSII

1

F

Hs

-1454

-1435

GGGAGTGGGATCTCTCCTTG

TSSII

2

F

Hs

-1308

-1289

TCTCCTCCATTCTGGGTCCT

TSSII

3

R

Hs

-1250

-1268

GGCTAGGGTCTGAATGCTCA

TSSII

4

R

Hs

-1201

-1220

GAGCCCAGCCTGAGTTGCTG

TSSII

5

F

Hs

-1135

-1115

CAGTCAGTCGTGGTCCCAAGG

TSSII

6

F

Hs

-1104

-1086

GCAGGTTTTTGGGTGAGTG

TSSII

7

F

Hs

-1038

-1018

GGCAGAGTCGACGGGAACAGT

TSSII

8

R

Hs

-1031

-1049

ACTGTTCCCGTCGACTCTGCC

intron

9

R

Hs

-913

-933

GTGGCTCTGGCCTCAGATGAT

intron

10

R

Hs

-842

-824

GCTAGGTGTCTGCTGCTCC

intron

11

R

Hs

-514

-534

AGCACAGAGAGTGGGCCTAAG

intron

12

F

Hs

-185

-164

CAGTGCCCTGTTACCGGGTCTC

TSSII&III

13

R

Hs

-119

-138

CCAGTCAGCCACTCTCGCGA

TSSII&III

14

R

Hs

-115

-134

ACAGCCAGTCAGCCACTCTC

cds

15

F

Hs

780

799

CTACAAGGTGTGGGCCAAGT

cds

16

R

Hs

952

933

CTTCCTCACCCTGCAGTAGC

TSSII

Mn1

F

Mm

-1061

-1042

GCAGGTTTTTGGGTGAATGT

TSSII

Mn2

F

Mm

-1003

-984

AGCCTACGGGAATAGGCACT

TSSII

Mn3

R

Mm

-981

-1000

CGCAGTGCCTATTCCCGTAG

  1. Hs - Homo sapiens; Mm - Mus musculus
  2. All human and mouse primers used in PCR experiments are listed. Their locations upstream from the start codon, direction (F represents the coding strand) and the features within the promoter near their locations are given. (The locations of primers are illustrated for human, Figure 2A and mouse, Figure 8A).