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Figure 5 | BMC Molecular Biology

Figure 5

From: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans-splicing group I introns

Figure 5

Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays. A) Drosophila S2 cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. B) Aag2 cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.

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