Figure 6
αDENV-GrpI constructs effectively target DENV-2 NGC. T-25 flasks containing D. melanogaster S2 cells (5 × 105) transiently transfected (A) or transformed (B) with αDENV-GrpI bicistronic constructs, were transfected with double-stranded DENV-2 target (+), or the negative control pUC57 empty vector (-). Resulting RNAs were analyzed by RT-PCR in the presence (+Rt) or absence (-Rt) of reverse transcriptase to insure that observed amplified products were derived from RNA. A PCR amplification product derived from a constructed spliced sequence control (Methods) is provided as size standard for each gel (DNA+Ctrl). Arrows indicate the predicted size of the principle splice products resulting from intron activity. The identity of splice product was confirmed by sequencing.