Figure 2
From: A tryptophan-rich peptide acts as a transcription activation domain
Assays of the transcriptional activities of the pentapeptide and its tandem repeats. (A) Transcriptional assays. The pentapeptide, WYDWW, and its tandem repeats were expressed as fusions to the specific DNA-binding protein, LexA, and the abilities of the resulting fusion proteins to turn on the reporter genes (LEU2 and lacZ), which are controlled by distinct LexA-responsive promoters, were tested. (B) Quantitative assays of β-gal activity. (C) Western blot analysis of the expressions of LexA fusion proteins. Upper panel, LexA fusion protein; lower panel, PGK (as a loading control). The numbers (circled) in A, B, and C represent the constructs shown in A.