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Figure 4 | BMC Molecular Biology

Figure 4

From: A tryptophan-rich peptide acts as a transcription activation domain

Figure 4

Assays of the transcriptional activities of W5, W7, and W9. (A) Transcriptional assays. W5, W7, and W9 were expressed as fusions to the specific DNA-binding protein, LexA, and the abilities of the resulting fusion proteins to turn on the reporter genes (LEU2 and lacZ), which are controlled by distinct LexA-responsive promoters, were tested. (B) Quantitative assays of β-gal activity. (C) Western blot analysis of the expressions of LexA fusion proteins. (D) RT-PCR. Relative levels of specific lexA mRNAs generated from each construct were determined by RT-PCR (28 cycles). Actin served as an internal control. "RNA control" denotes no reverse transcription for RNA prepared from W7. (E) Degradation assay. These constructs were expressed under the control of an inducible GAL1 promoter. Upper panel, LexA fusion protein; lower panel, PGK (as a loading control). T0, T0.5, T1, T2, T4, and T8mean 0, 0.5, 1, 2, 4, and 8 h post-induction, respectively. The numbers (circled) in A to E represent the constructs shown in A.

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