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Figure 1 | BMC Molecular Biology

Figure 1

From: Rrd1 isomerizes RNA polymerase II in response to rapamycin

Figure 1

Rrd1 is associated with the chromatin and interacts with Rpb1. A) Rrd1 is bound to chromatin. Whole cell extract (WCE), soluble (SOL) and chromatin (CHR) fractions were derived (see Methods) from the parent cells expressing either Rrd1-MYC, Swe1-MYC, Rad52-MYC or Apn1-MYC and the distribution of the MYC-tagged proteins was examined by Western blots. The data is representative of two independent analyses. B) Rpb1 pull-down of Rrd1. The 8WG16 antibodies were used to immunoprecipitate extracts from untreated (-) and rapamycin-treated (+) (200 ng/ml for 30 min) cells expressing either Rrd1-MYC or Swe1-MYC. The presence of Rrd1 in the immunoprecipitates was determined by Western blotting. C) Specificity of Rpb1 pull-down of GFP tagged proteins. The 8WG16 antibodies were used to immunoprecipitate extracts from cells expressing either of the following GFP tagged proteins: Imp2, Rrd1, Rad52 or Sit4. The presence of the GFP-tagged proteins in the immunoprecipitates was detected by Western blotting using GFP antibody.

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