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Figure 2 | BMC Molecular Biology

Figure 2

From: A mechanism for ramified rolling circle amplification

Figure 2

RAM reaction diagram. A) The circle represents a single-stranded circular DNA template molecule. A forward primer ("Fwd") is annealed to the template. "A-rvs", "B-rvs", and "C-rvs" labels refer to template sub-sequences that could serve as reverse primers. B) A RAM reaction in progress. The forward primer is displaced by its extension on the circular template to create the primary transcript that contains complementary binding sites for reverse primers. "A-rvs", "B-rvs", and "C-rvs" indicate alternate reverse-primer sequences corresponding to the template sub-sequences shown in A). The elongating primary transcript is labelled "extension locus". C) Three double-stranded products that would be formed in RAM reactions including the three reverse primers shown as examples in A) and B). The products, whose size depends on relative primer positions on the template, are formed after forward primer binding and extension on displaced extended reverse primer templates.

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