Skip to main content
Figure 1 | BMC Molecular Biology

Figure 1

From: Northern blotting analysis of microRNAs, their precursors and RNA interference triggers

Figure 1

Correlation between high-resolution northern blotting and deep sequencing results. A) Appropriately cropped representative northern pictures for endogenous mouse neuromiRs: miR-9, miR-9*, miR-29, miR-124, miR-132, miR-137, and myomiRs: miR-1, and miR-206 are shown. B) Comparative analysis of the relative distribution of miRNA length variants. The percentage of the various length variants of mouse miR-9, miR-9*, miR-29, miR-124, miR-132, miR-137, miR-1, and miR-206 observed in our high-resolution northern blot detections are shown as black bars; equivalent miRNAs identified by deep sequencing are shown as gray bars. Relative shares of miRNA length variants (denoted as nt) are calculated in percentage (%). Standard errors are from twelve independent northern blot samples for miR-9, miR-9*, miR-29, miR-124, miR-132, miR-137, from nine samples for miR-1, and from three samples for miR-206. One-nucleotide discrepancy between northern blotting and deep sequencing results observed in the case of miR-206 may be due to a difference in the migration rate of this miRNA, which probably results from its nucleotide composition.

Back to article page