Figure 6

Binding of Dge RecA (A) and Dmu RecA (B) to dsDNA in the absence and in the presence of heterologous ssDNA - gel mobility shift assays. Lane 1 - 600 bp PCR product, lane 2 - dsDNA binding products obtained in the absence of Mg²⁺ ions, lane 3 - dsDNA binding products obtained in the presence of 10 mM Mg²⁺ ions, lane 4 - 600 bp PCR product and 5'-end fluorescein-labeled oligo(dT)₃₅, lane 5 - DNA binding products obtained in the absence of Mg²⁺ ions, lane 6 - DNA binding products obtained in the presence of 10 mM Mg²⁺ ions.