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Figure 5 | BMC Molecular Biology

Figure 5

From: TRPC1 transcript variants, inefficient nonsense-mediated decay and low up-frameshift-1 in vascular smooth muscle cells

Figure 5

Expression of UPFs 1-3. (a-c) Example gels showing expression of mRNAs encoding UPF1 (a), UPF2 (b) and UPF3B (c) in HEK 293 and human saphenous vein VSMCs. Data are shown with (+) or without (-) reverse transcriptase (RT) reaction. (d) Mean data showing abundance of UPF1-3 mRNA species relative to β-actin in HEK 293 cells and VSMCs. (e) For a representative experiment, detection of UPF1 protein in HEK 293 cells and VSMCs. UPF1 was detected close to the expected molecular mass of 123 kDa. (f) Mean normalized data for experiments of the type illustrated in (e) (n = 3).

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