Figure 7

Inhibition of Ca²⁺ entry by over-expressed TRPC1 Δ8,9 in HEK 293 cells. Measurement of the intracellular Ca²⁺ concentration in transfected cells pretreated with 1 μM thapsigargin in zero Ca²⁺ solution before addition of 1.5 mM Ca²⁺ as indicated. Mean peak responses to 1.5 mM Ca²⁺ are shown for paired experiments, one comparing vector (n/N = 11/39) with vector containing the TRPC1-Δ8,9 insert (n/N = 13/35), the other comparing vector (n/N = 3/60) with vector containing full-length (FL) TRPC1 without the Δ8,9 deletion (n/N = 3/45). Test data are normalised to their respective controls (vector only).