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Figure 4 | BMC Molecular Biology

Figure 4

From: The resistance of the yeast Saccharomyces cerevisiae to the biocide polyhexamethylene biguanide: involvement of cell wall integrity pathway and emerging role for YAP1

Figure 4

Cytotoxicity assay to PHMB of yeast strains with deletion in oxidative stress response genes. (A) Spot test assay in YPD plates in the presence and absence of PHMB. (B) Determination of relative sulfhydryl groups in soluble proteins (PB) and non-proteins (NP) of molecules in the cell extract of BY4741 parental yeast cells and Δyap1 mutant strains in the presence of 0.005% PHMB and 5 mM H2O2.

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