Figure 1

F-actin-modifying chemicals attenuate retinoid receptor-mediated transcription. Jurkat cells were transfected with 5.0 ug TATADR1-Luc (A) or SP1-Luc plasmids (B). After 24 h, TATADR1-Luc transfected cells were treated for 16 h with 9-cis retinoic acid (1.0 uM) or vehicle in the presence of DMSO, or actin inhibitors latrunculin A (50 nM), swinholide A (2.5 nM) or jasplakinolide (50 nM). After 24 h, Sp1-Luc transfected cells were treated with DMSO or actin inhibitors for 16 h. Cells were harvested and luciferase activity measured as described in Experimental Procedures.