Skip to main content
Figure 6 | BMC Molecular Biology

Figure 6

From: SWI/SNF regulates the alternative processing of a specific subset of pre-mRNAs in Drosophila melanogaster

Figure 6

Depletion of the SWI/SNF core subunits changes the relative abundances of some mod(mdg4) mRNAs in S2 cells. S2 cells were treated with dsRNA for either Brm, Snr1 or Mor. Mock-treated cells were used as controls for microarray experiments (Moshkin et al. 2007), whereas the control cells in the validation experiments were treated in parallel with dsRNA for GFP. (A) The exon-intron organization of two mod(mdg4) mRNAs. The positions of the primer pairs used for qPCR are indicated by small arrows on top of each transcript. (B) Summary of the results derived from the microarray data. For each of the SWI/SNF core subunits, the histogram shows the average ratios between the abundance of each isoform in RNAi-treated cells compared to mock-treated cells (base on data from E-TABM-169). The bars represent average ratios calculated as in Figure 3. The error bars represent standard deviations. The stars mark transcript ratios that were affected by depletion of Brm, Snr1 or Mor according to the selection criteria described in the main text. (C), (D) and (E) Validation of the effects of the depletions on the relative abundances of the mod(mdg4) mRNAs, as in Figure 3. Either Brm, Snr1 or Mor was knocked down in S2 cells, and the relative abundance of each transcript was quantified by RT-qPCR using the primer pairs that target exon-exon junctions and downstream exons, as indicated in (A). The abundances of mod(mdg4)-RA and mod(mdg4)-RC were calculated relative to the abundance of a constitutive exon, as indicated in (A).

Back to article page