SWI/SNF regulates the alternative processing of a specific subset of pre-mRNAs in Drosophila melanogaster

BMC Molecular Biology

Table 2 Analysis of transcript stability in S2 cells

Gene	Transcript	RNAi	Half-life time¹	p-value²
Gpdh	RA	Brm	1.84 ± 0.41	0.31
		GFP	1.49 ± 0.11
		SNR1	1.98 ± 0.31	0.09
Gpdh	RB	Brm	3.32 ± 1.33	0.12
		GFP	1.39 ± 0.16
		SNR1	1.73 ± 0.24	0.12
Gpdh	RC	Brm	2.32 ± 0.20	0.02
		GFP	1.58 ± 0.17
		SNR1	2.01 ± 0.22	0.03
CG3884	RA	Brm	1.47 ± 0.20	0.43
		GFP	1.30 ± 0.13
		SNR1	1.40 ± 0.07	0.16
CG3884	RB	Brm	1.64 ± 0.11	0.32
		GFP	1.55 ± 0.02
		SNR1	1.64 ± 0.34	0.67
lola	RA	Brm	1.41 ± 0.16	0.40
		GFP	1.92 ± 0.62
		SNR1	1.47 ± 0.30	0.15
lola	RF	Brm	1.83 ± 0.15	0.10
		GFP	1.39 ± 0.12
		SNR1	1.63 ± 0.04	0.11
mod(mdg4)	RA	Brm	1.59 ± 0.31	0.38
		GFP	1.30 ± 0.15
		SNR1	1.35 ± 0.14	0.80
mod(mdg4)	RC	Brm	1.67 ± 0.47	0.29
		GFP	1.29 ± 0.04
		SNR1	1.60 ± 0.13	0.05

¹S2 cells were treated with dsRNA for Brm, GFP (control) or Snr1 for 24 hours, and treated with actinomycin D. RNA was purified after 0, 1.5 and 2.5 hours of treatment and quantified by RT-qPCR. The values are averages and standard deviations from three independent knock-down experiments.
² A paired, double-tailed Student's T-test was applied to compare the stabilities of each transcript in depleted and control cells.

ISSN: 1471-2199