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Table 1 Antibodies

From: Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays

Antibody

Catalog No.

Source

Manufacturer

Peptide immunogen

Flag M2

F 1804

Murine monoclonal

Sigma

Recognize a FLAG peptide sequence at the N-terminus, Met-N-terminus, C-terminus, or internal sites of a fusion protein

H2A.Z

39113

Rabbit polyclonal

Active Motif

C-terminus of human histone H2A.Z

H3K9/14Ac

06-599

Rabbit polyclonal

Millipore

KLH-conjugated peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 (ARTKQTAR[K*]STGG[K*]APRKQLC) where K* is acetylated.

H3K27m3

Ab6002

Mouse monoclonal

Abcam

Synthetic peptide derived from residues 1-100 of human histone H3, trimethylated at K27

mCpG

AMM99021

Mouse monoclonal

Aviva

5-methylcytosine conjugated to ovalbumin

mCpG

MAb-5MECYT

Mouse monoclonal

Diagenode

Raised against 5-methyl Cytidine conjugated to

ovalbumine

Pol II CTD (4H8)

Sc-47701

Mouse monoclonal

Santa Cruz

C-terminal domain peptide