pBRαLn+pACλcI | α1NTD | α1CTD | α2NTD | α2CTD |
|---|
α1NTD
| 171 ± 28 | 44 ± 1 |
1412 ± 323
| 53 ± 9 |
α1CTD
| 110 ± 13 | 95 ± 8 | 133 ± 5 | 99 ± 16 |
α2NTD
|
662 ± 26
| 47 ± 9 | 74 ± 4 | 49 ± 3 |
α2CTD
| 102 ± 3 | 101 ± 1 | 112 ± 6 | 86 ± 9 |
- Eight two-hybrid plasmids expressing bait (1st column) and prey (1st row) fusions of each α subunit domain were constructed and 16 pairwise combinations were tested in a reporter strain by measuring β-galactosidase activity (in Miller units). Each combination was tested at least three times independently. Mean and standard deviation values are presented. Three kinds of measurements were taken to determine background levels of β-galactosidase activity, which was found to be (in Miller units) 111 ± 26 in host reporter cells with no plasmids, 110 ± 9 in cells transformed with pBRαLN-α1NTD only, and 50 ± 7 in cells transformed with pACλcI-α1NTD only.
