Table 2 Yeast strains and plasmids used in this study.
From: Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene
Strain | Relevant genotype | Source or reference |
|---|---|---|
Isogenic to W303 | Â | |
EYL682 | MATa mpk1Δ::TRP1 ura3-1 trp1-1 leu2-3,112 ade2-1 ade3-1 his3 can1-100 lys2 (Gal+) | Elion lab collection |
EYL780 | MATa bar1Δ far1Δ ste11-4 ste12Δ::URA3 trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 | Elion lab collection |
EY957 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) | Elion lab collection |
EY1298 | MATa bar1Δ far1Δ ste11-4 trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 | Elion lab collection |
EYL4661 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste11Δ::Hygro | Elion lab collection |
EYL5328 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) pbs2Δ::Hygro | This study |
EYL5329 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) kss1Δ::HIS3 fus3Δ::Hygro pbs2Δ::Kan | This study |
EYL5330 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste12Δ::Hygro | This study |
EYL5331 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) bni1Δ::Hygro | This study |
EYL5332 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste7Δ::Hygro pbs2Δ::Kan | This study |
EYL5333 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cmk2Δ::Hygro | This study |
EYL5334 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ptk2Δ::Hygro | This study |
EYL5335 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cnb1Δ::Hygro | This study |
EYL5336 | MATa bar1Δ far1Δ trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 pbs2Δ::Hygro | This study |
EYL5337 | MATa bar1Δ far1Δ trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 cnb1Δ::Hygro | This study |
EYL5338 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) kss1Δ::HIS3 fus3Δ::Hygro | This study |
EYL5379 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ptk2Δ::Hygro cmk2Δ::Kan | This study |
EYL5380 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cmk2-tag(3-GFP)Kan | This study |
EYL5382 | MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) crz1Δ::Kan | This study |
S288c deletion strains in Figure 1 are isogenic to S288c BY4741 | [46] | |
BY4741 | MATa his3Δ1 ura3Δ0 leu2Δ0 met15-0 | [47] |
RG2468 | ste4Δ::KAN r leu2 ura3 his3 met15 | Research Genetics |
RG4038 | ste5Δ::KAN r leu2 ura3 his3 met15 | Research Genetics |
RG3439 | ste50Δ::KAN r leu2 ura3 his3 met15 | Research Genetics |
RG5271 | ste11Δ::KAN r leu2 ura3 his3 met15 | Research Genetics |
RG6981 | kss1 Δ::KANr leu2 ura3 his3 met15 | Research Genetics |
JAY408 | fus3Δ::KAN r ste5Δ::KAN r | [25] |
Isogenic to ∑1278b |  | |
L5528 | ura3-52, his3::hisG | [48] |
L5554 | ste5::LEU2, ura3-52, leu2::hisG | [48] |
Plasmids | Â | |
pYGU-11ΔN | pGAL1-STE11ΔN LEU2 CEN | [49] |
GAL1 vector | pGAL1, LEU2 CEN | Elion lab collection |
pYEE133 | pGAL1-STE12 TRP1 CEN | Elion lab collection |
GAL1 vector | pGAL1, TRP1 CEN | Elion lab collection |
pOO1 | pCMK2-lacZ, URA3, 2 μ | [19] |
EBL95 | pDL1460 pFUS1::ubiY-lacZ, URA3, 2 μ | Elion lab collection [50] |
EBL183 | PMC1-lacZ, URA3, 2 μ | [26] |
EBL185 | p DM5 FKS2 (-968-+6)-CYC1-lacZ, URA3, 2 μ | [26] |
EBL201 | FKS2 (-928-+1)-CYC1-lacZ, URA3, 2 μ | [14] |
EBL200 | FKS2 (-706-+1)-CYC1-lacZ, URA3, 2 μ | [14] |
EBL1258 | FKS2 (-540--375)-CYC1-lacZ, URA3, 2 μ | [15] |
EBL513 | YKL161c (MLP1)-lacZ, URA3, 2 μ | Elion lab collection |
EBL514 | YIL117c (PRM5)-lacZ, URA3, 2 μ | [51] |
EBL517 | pLGΔ-178 pCYC1-lacZ, URA3, 2 μ | [52] |