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Table 1 Oligonucleotides used for the cloning of different fragments of Dkl2 promoter in pGL3Basic and pGL3promoter vectors

From: Characterization of a proximal Sp1 response element in the mouse Dlk2 gene promoter

DNA FRAGMENT

OLIGONUCLEOTIDES

SEQUENCE

RESTRICTION ENZYMES

-1,090/+1*

Dlk2MluI-917U

5'-ATTACGCGTTTGTCAGGTGTAGGCGGTGGG-3'

Mlu I-Hind III

 

Dlk2HindIII-1L

5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'

 

-910/+1*

Dlk2MluI-722U

5'-GGCGACGCGTCAAATACACATATTGGGGTCTT-3'

Mlu I-Hind III

 

Dlk2HindIII-1L

5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'

 

-710/+1*

Dlk2MluI-522U

5'-TATACGCGTGGCAGGCTACCCAAAGGTGG-3'

Mlu I-Hind III

 

Dlk2HindIII-1L

5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'

 

-575/+1*

Dlk2MluI-400U

5'-TAGACGCGTAAGAAGCCCACAGAGAGCAGGC-3'

Mlu I-Hind III

 

Dlk2HindIII-1L

5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'

 

-375/+1*

Dlk2MluI-205U

5'-TATACGCGTTGGGTGAGGGGCAGAGTGG-3'

Mlu I-Hind III

 

Dlk2HindIII-1L

5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'

 

-212/+1*

Dlk2 Xho-212U

5'-TATCTCGAGGAAGGGAGGGGCGAAGAGC-3'

Xho I-Hind III

 

Dlk2HindIII-1L

5'-TATAAGCTTGCTGAGGCGACCCCGAGCG-3'

 

-1,090/-375

Dlk2MluI-917U

5'-ATTACGCGTTTGTCAGGTGTAGGCGGTGGG-3'

Mlu I-Hind III

 

Dlk2HindIII-184L

5'-GCTAAGCTT CCACTCTGCCCCTCACCCAC-3'

 

-1,090/-575

Dlk2MluI-917U

5'-ATTACGCGTTTGTCAGGTGTAGGCGGTGGG-3'

Mlu I-Hind III

 

Dlk2HindIII-379L

5'-TAGAAGCTTCGCCTGCTCTCTGTGGGCTTC-3'

 

-910/-375

Dlk2MluI-722U

5'-GGCGACGCGTCAAATACACATATTGGGGTCTT-3'

Mlu I-Hind III

 

Dlk2HindIII-184L

5'-GCTAAGCTT CCACTCTGCCCCTCACCCAC-3'

 

-710/-375

Dlk2MluI-522U

5'-TATACGCGTGGCAGGCTACCCAAAGGTGG-3'

Mlu I-Hind III

 

Dlk2HindIII-184L

5'-GCTAAGCTT CCACTCTGCCCCTCACCCAC-3'

 

+1/+177

Dlk2Xho I+1U

5'-ATTCTCGAGCCCAGATTCCCGAGTGCTCGGC-3'

Xho I-Hind III

 

Dlk2HindIII+177L

5'-ATGAAGCTTAGGGACCGCGTCCTCCTAGCTTC-3'

 

-212/+177*

Dlk2 Xho-212U

5'-TATCTCGAGGAAGGGAGGGGCGAAGAGC-3'

Xho I-Hind III

 

Dlk2HindIII+177L

5'-ATGAAGCTTAGGGACCGCGTCCTCCTAGCTTC-3'

 

-212/-427*

Dlk2 Xho-212U

5'-TATCTCGAGGAAGGGAGGGGCGAAGAGC-3

Xho I-Hind III

 

Dlk2HindIII+427L

5'-AATAAGCTT GTGGCCACCGCGCGGGAAC-3

 
  1. PCR reactions were performed under standard conditions (see Materials and Methods), except where indicated with an asterisk, in which the reactions were supplemented with 10% DMSO.